DETECTION OF MICROORGANISMS IN COMPRESSED GASES
Authors:Hans Zingre, R. Meier
Keywords:Microorganisms Under Pressure, Compressed Gases, ISO 14698, MAS-100 CG Ex, Nitrogen, Carbon-Dioxide, Argon, Oxygen, Pharmaceutical, Food Industry
Abstract:The International Standard Norm ISO 14698-1/21 demands the monitoring of bio contamination in
compressed gas systems which are used in pharmaceutical production processes. The norm describes the
detection of bio contamination by sampling and enumerating viable units with appropriate methods.
Two main questions were investigated:
Do microorganisms survive in compressed gas systems?
If they survive, what appropriate method has to be applied in order to sample and enumerate them?
13 different medical relevant species of microorganisms where grown and put under pressure from 10 to 170
bars. Their growth and behaviour under pressure and the effect of decompression has been analysed.
Based on the MAS-100 CG Ex (Microbiological Air Sampler for Compressed Gas) this presentation shows
that microorganisms can be measured and what trapdoors are to be avoided when dealing with colony
forming units under pressure
Click to Download PDF